Enhancing protein stability with retained biological function in transgenic plants

Induction of DrsB1-CBDAvr4 Recombinant Protein in Hairy and Adventitious Roots of T1 Transgenic Plants

Hairy and adventitious roots are efficient systems for expressing recombinant proteins. In the present study, the amount of DrsB1-CBDAvr4 recombinant protein in hairy and adventitious root systems was compared. To this end, the effect of different factors on the optimization of culture conditions to obtain adventitious and hairy roots was evaluated in three separate experiments by assessment of...

Compatibility of Insect-resistant Transgenic Plants with Biological Control

Transgenic Bt crops expressing proteins derived from Bacillus thuringiensis (Bt) currently are grown commercially in ten countries on over 20 million hectares. Assessing their environmental safety is a critical part of the regulatory approval process and product stewardship for Bt crops. The environmental safety testing process for Bt crops follows a standard risk assessment approach, and invol...

Enhancing protein stability with extended disulfide bonds.

Disulfide bonds play an important role in protein folding and stability. However, the cross-linking of sites within proteins by cysteine disulfides has significant distance and dihedral angle constraints. Here we report the genetic encoding of noncanonical amino acids containing long side-chain thiols that are readily incorporated into both bacterial and mammalian proteins in good yields and wi...

The Promyelocytic Leukemia Protein, PML, and Its Biological Function

Plants with Transgenic Plastids

Plant genetic engineering will likely contribute to the required continued increase in agricultural productivity during the coming decades. Moreover, plants can potentially provide inexpensive production platforms for pharmaceuticals and nutraceuticals. With the advent of technologies to alter the genetic information inside plastids (chloroplasts), a new attractive target for genetic engineerin...